RANKL and RANK are members of the TNF superfamily of ligands and receptors that play an important role in the regulation of specific immunity and bone turnover. RANK (receptor) was originally identified as a dendritic cell-membrane protein, which, by interacting with RANKL, augments the ability of dendritic cells. These dendritic cells then stimulate naïve T-cell proliferation in a mixed lymphocyte reaction, promote the survival of RANK+ T-cells, and regulate T-cell-dependent immune response. RANKL, which is expressed in a variety of cells, including osteoblasts, fibroblasts, activated T-cells and bone marrow stromal cells, is also capable of interacting with a decoy receptor called OPG. Binding of soluble OPG to sRANKL inhibits osteoclastogenesis by interrupting the signaling between stromal cells and osteoclastic progenitor cells, thereby leading to excess accumulation of bone and cartilage. Human RANKL is reactive on murine cells. Recombinant Human sRANKL is a 20.0 kDa polypeptide comprising the TNF-homologous region of RANKL (176 amino acid residues).

Source:E.coli

Synonyms:soluble Receptor Activator of NF-κB Ligand, TNFSF11, TRANCE (TNF-related activation-induced cytokine), OPGL, ODF (Osteoclast differentiation factor)

AA Sequence:MEKAMVDGSW LDLAKRSKLE AQPFAHLTIN ATDIPSGSHK VSLSSWYHDR GWAKISNMTF SNGKLIVNQD GFYYLYANIC FRHHETSGDL ATEYLQLMVY VTKTSIKIPS SHTLMKGGST KYWSGNSEFH FYSINVGGFF KLRSGEEISI EVSNPSLLDP DQDATYFGAF KVRDID

Purity:≥ 98% by SDS-PAGE gel and HPLC analyses.

Biological Activity:Determined by its dose-dependent ability to induce reporter gene in HT-29 NF-κB Luc reporter cells.

Calculated Molecular Weight:20 kDa

Accession Number:O14788

Gene ID:8600

Endotoxin:Endotoxin level is < 0.1 ng/ug of protein (< 1 EU/ug)

crossreactivity:

Human, Human + Cow, Mouse, Rat

References

PubMed

SDS

CoA 搜索

Product Line

Country Of Origin: USA

Not for human use.

Research Interest

细胞凋亡 骨/骨骼系统/软骨 癌症 糖尿病/体重调节 免疫系统 TNF超家族