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品牌 | ELK Biotechnology | |
基础属性 | 生产企业 | |
cas号 | ||
规格 | 96T | |
储存条件 | ||
纯度 | ||
密度 |
Product name: | Human SAM(S-Adenosyl Methionine) ELISA Kit |
Reactivity: | Human |
Alternative Names: | SAMe; SAM-e; S-Adenosylmethionine; S-(5'-Adenosyl)-L-Methionine Chloride |
Assay Type: | Competitive Inhibition |
Sensitivity: | 5.71 ng/mL |
Standard: | 1000 ng/mL |
Detection Range: | 15.63-1000 ng/mL |
Sample Type: | serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
Assay Length: | 2h |
Research Area: | Enzyme & Kinase; |
Uniprot ID: | P17707 |
Test principle: | This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human SAM protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human SAM. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human SAM in the samples is then determined by comparing the OD of the samples to the standard curve. |
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