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Human S100A8 ELISA Kit  

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货号CSB-E11833h

品牌CUSABIO

品牌类型无授权品牌

货期4天

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  • 详细信息
品牌 CUSABIO
基础属性 生产企业
cas号
规格 48T
储存条件
纯度
密度

产品详情

  • 产品描述：
    The human S100A8 ELISA Kit is engineered for accurate measurement of human S100A8 levels from samples including serum, plasma, or tissue homogenates. It uses the Sandwich-ELISA mechanism in combination with the enzyme-substrate chromogenic reaction to measure the S100A8 content in the sample. The color intensity is positively correlated with S100A8 content in the sample. The S100A8 concentration can be calculated according to the standard curve. This kit is tested with high sensitivity, strong specificity, good linearity, high precision and recovery, as well as lot-to-lot consistency. S100A8 binds to cell surface receptors that trigger signaling pathways to participate in the inflammatory process and plays a key role in multiple cellular processes, including cell survival, cell cycle progression, differentiation, proliferation, and cell migration. High expression of S100A8 has been found in various inflammation-related diseases like inflammatory bowel disease and solid cancers like bre...展开
  • 别名：
    60B8Ag ELISA Kit; AI323541 ELISA Kit; B8Ag ELISA Kit; BEE11 ELISA Kit; CAGA ELISA Kit; Calgranulin-A ELISA Kit; Calprotectin L1L subunit ELISA Kit; Calprotectin; included ELISA Kit; CFAG ELISA Kit; CGLA ELISA Kit; Chemotactic cytokine CP-10 ELISA Kit; CP-10 ELISA Kit; Cystic fibrosis antigen ELISA Kit; L1Ag ELISA Kit; Leukocyte L1 complex light chain ELISA Kit; MA387 ELISA Kit; MIF ELISA Kit; Migration inhibitory factor-related protein 8 ELISA Kit; MRP-8 ELISA Kit; Myeloid-related protein 8 ELISA Kit; Neutrophil cytosolic 7 kDa protein ELISA Kit; NIF ELISA Kit; p8 ELISA Kit; Pro-inflammatory S100 cytokine ELISA Kit; Protein S100-A8 ELISA Kit; S100 calcium binding protein A8 (calgranulin A) ELISA Kit; S100 calcium binding protein A8 ELISA Kit; S100 calcium-binding protein A8 ELISA Kit; S100A8 ELISA Kit; S100A8/S100A9 complex; included ELISA Kit; S10A8_HUMAN ELISA Kit; Urinary stone protein band A ELISA Kit
  • 缩写：
    S100A8
  • Uniprot No.：
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    1.25 ng/mL-80 ng/mL
  • 灵敏度：
    0.31 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Others
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
     
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human S100A8 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 89  
    Range % 83-95  
    1:2 Average % 95  
    Range % 91-99  
    1:4 Average % 95  
    Range % 88-101  
    1:8 Average % 98  
    Range % 91-102  
     
  • 回收率：
    The recovery of human S100A8 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 85 81-93  
    EDTA plasma (n=4) 95 93-102  
                 
                 
     
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected  
    80 2.945 2.962 2.954 2.800  
    40 2.567 2.504 2.536 2.382  
    20 1.676 1.799 1.738 1.584  
    10 0.849 0.887 0.868 0.714  
    5 0.441 0.411 0.426 0.272  
    2.5 0.263 0.251 0.257 0.103  
    1.25 0.201 0.211 0.206 0.052  
    0 0.155 0.152 0.154 　  
     
  • 数据处理：
  • 货期：
    3-5 working days
 

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