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| 品牌 | Proteintech | |
| 基础属性 | 生产企业 | |
| cas号 | ||
| 规格 | 50ul | |
| 储存条件 | -20 | |
| 纯度 | ||
| 密度 |
| Positive WB detected in | mouse liver tissue, rat liver tissue |
| Positive IP detected in | mouse liver tissue |
| Positive IHC detected in | human liver cancer tissue, human placenta tissue, human pancreas cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| APPLICATION | DILUTION |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Sample-dependent, check data in validation data gallery | |
| WB | See 9 publications below |
| IHC | See 6 publications below |
12589-1-AP targets CDO1 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.
| Tested Applications | IHC, IP, WB,ELISA |
| Cited Applications | IHC, WB |
| Tested Reactivity | Human, Mouse, Rat, Pig |
| Cited Reactivity | Human, Mouse, Rat |
| Immunogen | CDO1 fusion protein Ag3294 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | cysteine dioxygenase, type I |
| Synonyms | CDO, CDO I, CDO1, Cysteine dioxygenase type 1, cysteine dioxygenase, type I |
| Calculated molecular weight | 200 aa, 23 kDa |
| Observed molecular weight | 23 kDa |
| GenBank accession number | BC024241 |
| Gene symbol | CDO1 |
| Uniprot | Q16878 |
| Gene ID (NCBI) | 1036 |
| RRID | AB_10638145 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
CDO1(cysteine dioxygenase type 1) is also named as CDO and belongs to the cysteine dioxygenase family. It is an enzyme that adds molecular oxygen to the sulfur of cysteine, converting the thiol to a sulfinic acid known as cysteinesulfinic acid (3-sulfinoalanine) and CDO1 is one of the most highly regulated metabolic enzymes responding to diet(PMID:19011731). The expression of CDO can significantly decrease the level of intracellular cysteine and this reduction is also associated with a decrease in total glutathione levels(PMID:17327371). Cysteine dioxygenase (CDO) plays a critical role in the regulation of cellular cysteine concentration andmultiple forms of CDO (23 kDa, 25 kDa, and 68 kDa) have been claimed based upon separation and detection using SDS-PAGE/western blotting(PMID: 14752623).
| PRODUCT SPECIFIC PROTOCOLS | |
|---|---|
| WB protocol for CDO1 antibody 12589-1-AP | Download protocol |
| IHC protocol for CDO1 antibody 12589-1-AP | Download protocol |
| IP protocol for CDO1 antibody 12589-1-AP | Download protocol |
| STANDARD PROTOCOLS | |
|---|---|
| Click here to view our Standard Protocols |
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