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货号ab8226
品牌Abcam
品牌类型无授权品牌
货期电话联系
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| 品牌 | Abcam | |
| 基础属性 | 生产企业 | |
| cas号 | ||
| 规格 | 10 µg Trial Size | |
| 储存条件 | ||
| 纯度 | ||
| 密度 |
Anti-beta Actin抗体[mAbcam 8226] - Loading Control
参阅全部 beta Actin 一抗
小鼠单克隆抗体[mAbcam 8226] to beta Actin - Loading Control
Mouse
Does not cross-react with adult cardiac, smooth, or skeletal muscle actin. The immunogen used for this product shares 77% homology with Gamma actin/actin cytoplasmic 2. Cross-reactivity with this protein has not been confirmed experimentally.
适用于: ICC/IF, IHC-P, WBmore details
与反应: Mouse, Rat, Human
预测可用于: Sheep, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Pig, Monkey, Zebrafish, African green monkey, Chinese hamster, Armenian hamster
Synthetic peptide corresponding to Human beta Actin aa 1-100 conjugated to keyhole limpet haemocyanin (Sulfosuccinimidyl 4-N-maleimidomethyl-cyclohexane-1-carboxylate (Sulfo-SMCC)).
Database link: P60709
(Peptide available as ab13772)
Western blot protocol advice:
We recommend blocking with 2-5% BSA as we have found that use of 5% milk significantly reduces the band intensity for beta actin. Please see the comparison data in the images section. If milk block is required, we recommend using ab8224 mouse monoclonal [mAbcam 8224] to beta actin. Contact our Scientific Support team for more information or advice.
This antibody clone [mAbcam 8226] is manufactured by Abcam.
If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Abpromise™承诺保证使用ab8226于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF | (17) |
Use a concentration of 5 µg/ml. |
| IHC-P | (1) |
Use a concentration of 0.1 - 0.5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
| WB | (100) | Use a concentration of 1 µg/ml. Predicted molecular weight: 42 kDa.Can be blocked with Human beta Actin peptide (ab13772).
We recommend blocking using 2-5% BSA as we have found that use of 5% milk significantly reduces the band intensity for beta actin. Please refer to the images section for the blocking comparison data. Read More |
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