产品名称: | ACTB Monoclonal Antibody |
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货号: | AC026 |
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宿主: | Rabbit |
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同种型: | IgG |
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免疫原: | Recombinant protein of human β-actin |
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基因ID: | 60 |
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Swiss Prot: | P60709 |
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别名: | BRWS1; PS1TP5BP1 |
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反应物种: | Human, Mouse, Rat, Chicken, Zebrafish, Pig, Oryza sativa |
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验证应用: | WB, IHC |
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推荐稀释比: | WB 1:50000 - 1:200000 IHC 1:200 - 1:2000 |
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理论分子量: | 41kDa |
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实际分子量: | 43kDa |
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阳性样本: | NIH/3T3, TE-1, 293T, SW480, Mouse brain, Mouse testis, Mouse lung, Rat brain, HeLa |
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细胞定位: | Cytoplasm,cytoskeleton |
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存储缓冲液: | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
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检测图片: | | Western blot - β-actin antibody (AC026) Western blot analysis of extracts of various cell lines, using β-actin antibody (AC026) at 1:100000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
| Western blot - β-actin antibody (AC026) Western blot analysis of extracts of HeLa cells, using β-actin antibody (AC026) at 1:10000-1:2560000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s. |
| Immunohistochemistry - β-actin antibody (AC026) Immunohistochemistry of paraffin-embedded human breast cancer using β-actin antibody (AC026) at dilution of 1:100 (20x lens). |
| Immunohistochemistry - β-actin antibody (AC026) Immunohistochemistry of paraffin-embedded mouse liver using β-actin antibody (AC026) at dilution of 1:100 (20x lens). |
| Immunohistochemistry - β-actin antibody (AC026) Immunohistochemistry of paraffin-embedded rat kidney using β-actin antibody (AC026) at dilution of 1:100 (20x lens). |
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