LiCl   目录号 GC12077   Purity: >99.00%

used to precipitate RNA

产品描述
IC50: N/A

LiCl is a typical ionic compound, though the small size of the Li+ ion makes its properties not seen for other alkali metal chlorides, such as the extraordinary solubility in polar solvents and hygroscopic properties.

In vitro: Though precipitation with a monovalent cation or alcohol is much more widely used, LiCl has been frequently used to precipitate RNA. LiCl precipitation gives various advantages over other methods: LiCl does not precipitate DNA, protein and carbohydrate efficiently. LiCl can be also used for removing translation inhibitors or cDNA synthesis from RNA preparations. LiCl can be also used as a simple rapid method for recovering RNA from in-vitro transcription reactions [1]. In addition, a method for isolation of large, translationally active RNA samples has been presented. In this study, the procedure included homogenization of samples in 5 m guanidine monothiocyanate followed by direct precipitation of RNA from the guanidinium by 4 m LiCl. The advantages of this method include simplicity, speed, avoidance of ultracentrifugation, and its applicability to large numbers of samples. The method yielded large mRNA precursors up to 10 kb [2].

In vivo: Currently, there is no animal data reported.

Clinical trial: Up to now, LiCl has not been used clinically.

References:
[1] BARLOW JJ, MATHIAS AP, WILLIAMSON R, GAMMACK DB.  A simple method for the quantitative isolation of undegraded high molecular weight ribonucleic acid. Biochem Biophys Res Commun.1963 Sep 10;13:61-6.
[2] Cathala, G. , Savouret, J., Mendez, B., West, B. L., Karin, M., Martial, J. A., and Baxter, J. D. (1983). "A Method for Isolation of Intact, Translationally Active Ribonucleic Acid".DNA.2(4): 329–335.

Chemical Properties
Cas No.    7447-41-8    SDF    
化学名    lithium chloride
Canonical SMILES    [Cl-].[Li+]
分子式    ClLi    分子量    42.39
溶解度    ≥ 4.2mg/mL in H2O    储存条件    Store at RT
General tips    For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.
Shipping Condition    Evaluation sample solution : ship with blue ice
All other available size: ship with RT , or blue ice upon request